doc. RNDr. Soňa Kucharíková, PhD.

E-mail
sona.kucharikova@truni.sk

Pôsobí ako odborná asistentka Katedry Laboratórnych vyšetrovacích metód v zdravotníctve na Fakulte zdravotníctva a sociálnej práce Trnavskej univerzity v Trnave. Vyučuje predmety Mikrobiológia, Aplikovaný výskum, Výskum v laboratórnej medicíne a Tvorbu odbornej publikácie. Momentálne pôsobí ako vedeckovýskumný pracovník v Pavilóne klinických a laboratórnych disciplín. Venuje sa najmä problematike biofilmov spôsobených patogénnymi kvasinkami Candida albicans a Candida glabrata a baktériami Staphylococcus aureus a Staphylococcus epidermidis. Taktiež sa zaoberá problémom narastajúcej rezistencie voči antibiotikám a antifungálnym látkam.

Rok narodenia: 1983
Rok získania PhD.: 2010 (Prírodovedecká fakulta UK v Bratislave), 2011 (Katolícka univerzita Leuven, Belgicko)

 

Publikácie

  1. Rogiers Ona., Holtappels M., Siala W., Lamkanfi M., Van Bambeke F., Lagrou K., Van Dijck P. and Kucharíková S. (2018) Anidulafungin increases anti-bacterial activity of tigecycline in polymicrobial Candida albicans-Staphylococcus aureus biofilms on intraperitoneally implanted foreign bodies. Accepted for publication in Journal of Antimicrobial Chemotherapy. doi: 10.1093/jac/dky246.
  2. Vande Velde G., Kucharíková S., Van Dijck P. and HimmelreichU. (2018) Bioluminescence imaging increases in vivo screening efficiency for antifungal activity against device-associated Candida albicans biofilms. Accepted for publication in International Journal of Antimicrobial Agents 52(1). 42-51.
  3. Holtappels M, Swinnen E, De Groef L, Wuyts J, Moons L, Lagrou K, Van Dijck P, Kucharíková S. (2017) Antifungal Activity of Oleylphosphocholine on In Vitro and In Vivo Candida albicans Biofilms. Antimicrobial Agents and Chemotherapy 62(1). doi: 10.1128/AAC.01767-17. 
  4. Cools TL, Vriens K, Struyfs C, Verbandt S, Ramada MHS, Brand GD, Bloch C Jr, Koch B, Traven A, Drijfhout JW, Demuyser L, Kucharíková S, Van Dijck P, Spasic D, Lammertyn J, Cammue BPA, Thevissen K. (2017) The antifungal plant defensin HsAFP1 is a phosphatidic acid-interacting peptide inducing membrane permeabilization. Frontiers in Microbiology 8:2295. doi: 10.3389/fmicb.
  5. Cools TL, Struyfs C, Drijfhout JW, Kucharíková S, Lobo Romero C, Van Dijck P, Ramada MHS, Bloch C Jr, Cammue BPA, Thevissen K. (2017) A linear 19-Mer plant defensin-derived peptide acts synergistically with caspofungin against Candida albicans biofilms. Frontiers in Microbiology 8:2051. doi: 10.3389/fmicb.
  6. Kong EF, Tsui C, Kucharíková S, Van Dijck P, Jabra-Rizk MA. (2017) Modulation of Staphylococcus aureus response to antimicrobials by the Candida albicans quorum sensing molecule farnesol. Antimicrobial Agents and Chemotherapy 61(12). doi: 10.1128/AAC.01573-17.
  7. Siala W, Kucharíková S, Braem A, Vleugels J, Tulkens PM, Mingeot-Leclerq MP, Van Dijck P, Van Bambeke F. (2016) The antifungal caspofungin increases fluoroquinolone activity against Staphylococcus aureus biofilms by inhibiting N-acetylglucosamine transferase. Nature Communications 7:13286. 2016.
  8. Kong EF, Tsui C, Kucharíková S, Andes D, Van Dijck P, Jabra-Rizk MA. (2016) Commensal Protection of Staphylococcus aureus against Antimicrobials by Candida albicans Biofilm Matrix. MBio 7(5):e01365-16. doi: 10.1128/mBio.01365-16.
  9. Peeters E, Hooyberghs G, Robijns S, Waldrant K, De Weerdt A, Delattin N, Liebens V, Kucharíková S, Tournu H, Verstraeten N, Dovgan B, Girandon L, Fröhlich M, De Brucker K, Van Dijck P, Michiels J, Cammue BP, Thevissen K, Vanderleyden J, Van der Eycken E, Steenackers HP. (2016) Modulation of the substitution pattern of 5-aryl-2-aminoimidazoles allows fine-tuning of their anti-biofilm activity spectrum and toxicity. Accepted in Antimicrobial Agents and Chemotherapy. 2016. pii: AAC.00035-16. 
  10. Gerits E, Kucharíková S, Van Dijck P, Erdtmann M, Krona A, Lövenklev M, Fröhlich M, Dovgan B, Impellizzeri F, Braem A, Vleugels J, Robijns SC, Steenackers HP, Vanderleyden J, De Brucker K, Thevissen K, Cammue BP, Fauvart M, Verstraeten N, Michiels J. (2016) Antibacterial activity of a new broad-spectrum antibiotic covalently bound to titanium surfaces. Journal of Orthopaedic Research. doi: 10.1002/jor.23238.
  11. Formosa-Dague C, Feuillie C, Beaussart A, Derclaye S, Kucharíková S, Lasa I, Van Dijck P, Dufrêne YF. (2016) Sticky Matrix: Adhesion Mechanism of the Staphylococcal Polysaccharide Intercellular Adhesin. ACS Nano 10(3):3443-52.
  12. Kucharíková S, Gerits E, De Brucker K, Braem A, Ceh K, Majdič G, Španič T, Pogorevc E, Verstraeten N, Tournu H, Delattin N, Impellizzeri F, Erdtmann M, Krona A, Lövenklev M, Knezevic M, Fröhlich M, Vleugels J, Fauvart M, de Silva WJ, Vandamme K, Garcia-Forgas J, Cammue BP, Michiels J, Van Dijck P, Thevissen K. (2016)  Covalent immobilization of antimicrobial agents on titanium prevents Staphylococcus aureus and Candida albicans colonization and biofilm formation. Journal of Antimicrobial Chemotherapy 71(4):936-45.
  13. El-Kirat-Chatel S., Beaussart A., Derclaye S., Alsteens D., Kucharíková S., Van Dijck P., Dufrêne. (2015) Force nanoscopy of hydrophobic interactions in the fungal pathogen Candida glabrata. ACS Nano 9(2):1648-55.
  14. Kucharíková S., Vande Velde G., HimmelreichU. and Van Dijck P. (2015) Candida  albicans biofilm development on medically-relevant foreign bodies in a mouse subcutaneous model followed by bioluminescence imaging. J Vis Exp 95. doi: 10.3791/52239.
  15. Kong E.*, Kucharíková S.*, Van Dijck P., Peters B. M., Shirtliff M. E., Jabra-Rizk M. A. (2015). Clinical implications of oral candidiasis: host tissue damage and disseminated bacterial disease. Infect Immun83(2):604-13. * equal contribution.
  16. Kucharíková S., Neirinck B., Sharma S., Vleugels J., Lagrou K. and Van Dijck P. (2015) In vivo Candida glabrata biofilm development on foreign body in a rat subcutaneous model. J Antimicrob Chemother 70(3):846-56
  17. De Cremer K., Delattin N., De Brucker K., Peeters A., Kucharíková S., Gerits E., Verstraeten N., Michiels J., Van Dijck P., Cammue B. P., Thevissen K. (2014) Oral administration of the broad-spectrum antibiofilm compound toremifene inhibits Candida albicans and Staphylococcus aureus biofilm formation in vivo. Antimicrob Agents Chemother 58(12):7606-10.
  18. Vande Velde G., Kucharíková S., Van Dijck P. and HimmelreichU. (2014) Bioluminescence imaging of fungalbiofilm development in live animals. Methods Mol Biol 1098, 153-167.
  19. 19.      Vande Velde G., Kucharíková S.*, Schrevens S., HimmelreichU. and Van Dijck P. (2014) Towards non-invasive monitoring of pathogen-host interactions during biofilm formation by Candida albicans using in vivo bioluminescence. Cellular Microbiology 16(1)115-130. * equal contribution
  20. Beaussart A., Herman P., El-Kirat-Chatel S., Lipke P.N., Kucharíková S., Van Dijck P. and Dufrêne. (2013) Single-cell force spectroscopy of the medically-important Staphyloccocus epidermidis-Candida albicans interaction. Nanoscale 5(22):10894-900.
  21. Kucharíková S., Spriet I., Maertens J., Van Dijck P. and Lagrou K. Activities of systemically administred echinocandins against in vivo mature Candida albicans biofilms developed in a subcutaneous rat model. (2013) Antimicrob Agents Chemother 57(5), 2365-2368, 2013.
  22. Beaussart A., Alsteens D., El-Kirat-Chatel S., Lipke P.N., Kucharíková S., Van Dijck P. and Dufrêne. (2012) Single-molecule imaging and functional analyses of Als adhesins and mannans during Candida albicans morphogenesis. ACS Nano 6(12) 10950-10964.
  23. BinkA.* KucharíkováS.*, Vleugels J., Neirinck B. Van Dijck P., CammueB. P.A. and Thevissen K. (2012) The non-steroidal anti-inflammatory drug Diclofenac potentiates the in vivo activity of Caspofungin against Candida albicans biofilms. J Infect Dis 206(11) 1790-1797. * equal contribution
  24. Shahrooei M., Hira V., Khodaparast L., Khodaparast L., Stijlemans B., Kucharíková S., Burghout P., Hermans P. and Van Eldere J. (2012) Vaccination with SesC decreases Staphylococcus epidermidis biofilm formation. Infect Immun 80, 3660-3668.
  25. FioriA., KucharíkováS., Govaert G., CammueB. P. A., Thevissen K. and Van DijckP. (2012) The heat-induced molecular disaggregase Hsp104 of Candida albicans plays a role in biofilm formation and pathogenicity in the worm infection model. Eukaryotic Cell 11(8), 1012-1020.
  26. BinkA., GovaertG., VandenboschD., KucharíkováS., Coenye T., NelisH., Van Dijck P., CammueB. P.A. and Thevissen K. (2012) Efg1 Protects Candida albicans Biofilms to Miconazole In vitro and In vivo. J Med Microbiol 61(Pt 6), 813–819.
  27. Kucharíková S., Tournu H., Lagrou K., Van Dijck P.and Bujdáková H. (2011) Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and its susceptibility to echinocandins. J Med Microbiol 60, 1261–1269.
  28. Kucharíková S., Tournu H., Holtappels M. Van Dijck P., Lagrou K. (2010) In vivo efficacy of anidulafungin against mature Candida albicans biofilms in a novel rat model of catheter-associated candidiasis. Antimicrob Agents Chemother 54 (10):4474-4475.
  29. Nailis H., Kucharíková S., Řičicová M., Van Dijck P., Deforce D., Nelis H. and Coenye T. (2010) Real-time PCR expression profiling of genes encoding potential virulence factors in Candida albicans biofilms: identification of model-dependent and -independent gene expression. BMC Microbiology10: 114 doi: 10.1186/1471-2180-10-114
  30. Řičicová M.*, Kucharíková S.*, Tournu H.*, Hendrix J., Bujdáková H.,  Van Eldere J., Lagrou K. and Van Dijck P. (2010) Candida albicans biofilm formation in a new in vivo rat model. Microbiology 156 (Pt3): 909-919. * equal contribution
  31. Kucharíková S., Van Dijck P., Lisalová M. and Bujdáková H. (2010) Effect of antifungals on itraconazole resistant Candida glabrata. Cent Eur J Biol 5(3): 318-323.
  32. Borecká-Melkusová S., Moran G. P., Sullivan D. J., Kucharíková S., Chorvát Jr D., Bujdáková H. (2009) The expression of genes involved in the ergosterol biosynthesis pathway in Candida albicans and Candida dubliniensis biofilms exposed to fluconazole. Mycoses 52:118-128.
  33. Helena Bujdáková, Ema Paulovičová, Silvia Borecká-Melkusová, Juraj Gašperík, Soňa Kucharíková, Anna Kolecka, Claudia Lell, Dorthe B. Jensen, Reinhard Würzner, Dušan Chorvát and Iva Pichová. (2008) Partial purification and immunological characterization of the 45 kDa cell surface antigenof Candida albicans and investigation of its potential role in adherence. J Med Microbiol 57:1466-1472.

 

Vedecké granty: hlavný navrhovateľ a riešiteľ

  • FWO postdoctoral grant 12D3516 N (2015-2018) “Candida albicans-Staphylococcus epidermidis dual-species biofilm development: Molecular characterization of their interaction and investigation of the role of small secreted molecules in this process”.
  • FWO postdoctoral grant 12D3516 N (2012-2015) “Candida albicans-Staphylococcus aureus dual-species biofilm: Molecular characterization of their interaction with a focus on methionine metabolism”.
  • KU Leuven Postdoctoral grant, PDMK/11/089 (2011-2012) s názvom: “Development of novel antimicrobial peptides to combat Candida albicans biofilm formation”.
  • FWO Krediet aan Naavorser grant 1527216N (2015)“Zebrafish embryos as a unique model host organism to study in vivo Candida albicans biofilm development” na kúpu systému “Microfluidics”.
  • Pfizer WI189957 (2014) research grant “Comparison of echinocandins against in vivo Candida albicans biofilms”.
  • ESCMID research grant 2013 (European Society of Clinical Microbiology and Infectious Diseases) “First study dedicated to detailed characterization of mature Candida glabrata biofilm development in a subcutaneous rat model and its susceptibility to fluconazole and echinocandin drugs (micafungin, caspofungin and anidulafungin)”.
  • CREA 13/008, KU Leuven research grant (2013) s názvom: “Zebrafish as a novel model host for morphological and undiscovered immunological characteristics of in vivo Candida albicans biofilms”.
  • Research grant (2008)from Comenius University in Bratislava UK/183/2008
  • Research project Action Austria-Slovakia for doctoral students (2007) ”Interaction of mammalian immune cells with Candida albicans biofilms”
  • FEMS research grant (2007): “In vivo Candida albicans biofilm formation in a rat subcutaneous model”.
  • Research grant 4/2006 (2006) of Faculty of Natural Sciences, Comenius University in Bratislava.

 

English CV (PDF, 140.96 KB)